Colloids and Nanomedicines 2012
We will visit this conference with our partner LOT-Oriel.
We especially recommend the posters below that will be presented at this conference, and would be happy to discuss their content or other questions in our booth.
You are of course welcome to contact us in advance with any questions or requests.
Contact person: Raimund Sauter
Recommended posters
A Nanoparticle – Lipid Membrane Interaction Platform
Sofia Svedhem*, Rickard Frost, Laura De Battice, Angelika Kunze, and Bengt Kasemo
Dept. of Applied Physics, Chalmers University of Technology, SE 412 96 Göteborg, Sweden
*sofia.svedhem@chalmers.se
In vitro characterization of nanoparticles is becoming increasingly important due to the rapid development of novel nanoparticle formulations for applications in the field of Nanomedicine. Commonly, nanoparticles are simply characterized with respect to their size and zeta potential, and additional descriptors will be needed to develop useful nanoparticle structure – activity relationships. We are developing methodology to study interactions between nanoparticles and model interfaces using surface-sensitive analytical techniques. Our main focus is on model lipid membranes which are formed onto a sensor surface. Examples where this platform has been used will be described, covering e.g. polyelectrolyte complexes for drug delivery applications [1], and titania nanoparticles [2]. Notably, it was shown by a combination of quartz crystal microbalance (QCM-D) and atomic force microscopy (AFM) that titania nanopartilces induce holes into supported lipid membranes of certain compositons. An ion-mediated mechanism for these observations will be discussed.
[1] (a) Frost, R., Grandfils, C., Cerda, B., Kasemo, B., and Svedhem, S. The interaction of polycationic nanoparticles for drug delivery with biomimetic model membranes, Journal of Biomaterials and Nanobiotechnology, 2:181-193, 2011; (b) Frost, R., Coué, G., Engbersen, J.F.J., Zäch, M., Kasemo, B., and Svedhem, S. Bioreducible insulin-loaded nanoparticles and their interaction with model lipid membranes, Journal of Colloids and Interfaces, 362:575-583, 2011
[2] Zhao, F., Perez Holmberg, J., Frost, R., Abbas, Z, Kasemo, B., Hassellöv, M., and Svedhem, S. TiO2 nanoparticle induced membrane damage, submitted.
Surface based analysis of nanomaterials´ action on cellular processes
Rickard Frost, Elisabeth Norström, Lovisa Bodin, Joachim Sturve, Margareta Wallin, and Sofia Svedhem
Recently, we have applied the quartz-crystal microbalance with dissipation monitoring (QCM-D) technique to study intracellular processes. Specifically, Xenopus laevis melanophores have been investigated with respect to melanosome (pigment granule) translocation upon addition of hormones stimulating transport, drugs targeting the cytoskeleton and manufactured nanomaterials. It has been shown that the reversible aggregation and dispersion of melanosomes, that are intracellular transport processes occurring along microtubules and actin filaments, generate characteristic QCM-D responses. From this point we have studied the effects of drugs disrupting the cytoskeleton, to learn what information that lies in the QCM-D data, and the action of nanomaterials on these transport processes.
We believe that the described methodology will become an important tool to evaluate and characterize the action (toxicity or preferred functionalities) of engineered nanomaterials, e.g. drug delivery systems.
Conference website: www.colloidsandnanomedicines.com
